The Super Mario Bros. Super Show! was the mixed live action and animated series that aired on weekday syndication. The first part of it was live-action and showed Mario (played by Captain Lou Albano) and Luigi (Danny Wells) living in Brooklyn, where they would often be visited by celebrity guest stars. Occasionally, the main actors would be playing guest stars themselves, forcing their regular characters to leave when it came time for their other characters to show up.

The second part was a cartoon based on the Super Mario Bros. and Super Mario Bros. 2 video games, where Mario, Luigi, Princess Toadstool, and Toad battle against King Koopa, usually in a parody of something. Getting into the spirit of these parodies, Koopa usually had a different outfit and/or name for each one. Interestingly, Wart, the main antagonist of the second game, was never in any of the episodes, yet practically all of his minions managed to appear; this seems to suggest that Wart is perhaps in alignment with King Koopa. Like most 1980s cartoons, Koopa would prolong the series' run by using a Sub-Space potion to escape from his adversaries (despite the fact that they could easily catch him). The Super Mario Bros. cartoon was shown on Mondays through Thursdays only.

On Fridays, the show would air the Legend of Zelda cartoons based on the game of the same name, in which Link and Princess Zelda are fighting against the forces of Ganon.

After the first year of syndication the live action segments were replaced with a small group of people called club mario who would talk about the show while airing the cartoon.