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Bio-Dome is a 1996 American comedy film directed by Jason Bloom. Bio-Dome was produced by Motion Picture Corporation of America on a budget of $15 million and was distributed theatrically by Metro-Goldwyn-Mayer.

The plot of the film revolves around two clumsy, dim-witted slackers who, while on a road trip, look for a toilet stop in what they believe is a shopping mall, which in fact turns out to be a "bio-dome", a form of a closed ecological system in which five scientists are to be hermetically sealed for a year. The film has themes of environmentalism, combined with substance abuse, sexual innuendo, and toilet humor.

The film stars Stephen Baldwin and Pauly Shore, and has cameo appearances by celebrities such as Roger Clinton, Kylie Minogue, Patricia Hearst, and Rose McGowan. Jack Black and Kyle Gass first came to global attention in Bio-Dome in which they performed together as Tenacious D on-screen for the first time.

The film grossed $13 million at the box office in North America. Bio-Dome was widely panned by mainstream critics.

On December 18, 2013, Stephen Baldwin appeared on Mancow Muller's radio/TV show, confirming that he is in talks with Pauly Shore about making a sequel to the film revolving around the children of their characters Bud and Doyle.